Journal: Cell Death & Disease

Article Title: Ferroptosis involves in intestinal epithelial cell death in ulcerative colitis

doi: 10.1038/s41419-020-2299-1

Figure Lengend Snippet: a Cell death of HCoEpiC cells with or without RSL3 (20 μ m ) challenged for 8 hours was measured by PI staining, positive signal was stained in red (Scale: 50 μm). b ROS of HCoEpiC cells with or without RSL3 treatment was detected by C11-BODIPY staining, positive signal was stained in red (Scale: 50 μm). c Western blotting analysis of p-eIF2α, eIF2α, FTL, and FTH in cells with RSL3 challenged in the indicated times. β-actin was used as the loading control. d Relative mRNA level of GPR78, ATF4, CHOP, PTGS2, FTL, and FTH in HCoEpiC cells were measured by real-time PCR. Cells were treated with or without RSL3 for 8 h, GSK2606414 (GSK 414, 1 μ m ) was added to the cells 30 mins before RSL3. e Western blotting analysis of p-eIF2α, eIF2α, FTL, and FTH in cells with or without RSL3 and GSK 414 treatment. β-actin was used as the loading control. f Cell death of indicated cells was tested through PI staining (Scale: 50 μm). g ROS of indicated cells was detected by C11-BODIPY staining (Scale: 50 μm). Statistical analyses were performed with Student’s t tests (two groups) or one-way ANOVA (more than two groups).

Article Snippet: To treat mice with GSK2606414 (GSK 414) in vivo, the mice were administered either GSK 414 (Selleck, Shanghai, China, suspended in vehicle solution containing 0.5% hydoxypropylmethyl cellulose and 0.1% Tween 80 in water at pH 4.8, 50 mg/kg body weight) , or vehicle solution by oral gavage daily during DSS administration.

Techniques: Staining, Western Blot, Control, Real-time Polymerase Chain Reaction

Journal: Cell Death & Disease

Article Title: Ferroptosis involves in intestinal epithelial cell death in ulcerative colitis

doi: 10.1038/s41419-020-2299-1

Figure Lengend Snippet: Mice were treated with GSK2606414 (GSK 414) or vehicle solution. a Comparison of the disease activity index between colitis mice with or without GSK 414 challenged. * P < 0.05 versus mice from vehicle group, n = 8 in each group. b Representative images from H&E staining colonic sections from indicated mice (Scale: 100 μm). c Histologic scores were determined according to H&E-stained sections. d MDA levels were detected in colonic epithelial tissue from DSS-treated mice with or without GSK 414 challenged. e PI-labeled necrotic cell in colonic epithelial cells were analyzed by flow cytometry. f Iron levels were measured in colonic epithelial tissue from DSS-treated mice with or without GSK 414 administration. g Double immunofluorescent staining for p-eIF2α and FTH in the indicated colonic sections. Nuclei was stained with DAPI in blue. p-eIF2α was stained in green, and FTH was visualized in red. The merging positive signals of p-eIF2α and FTH were visualized in yellow (Scale: 50 μm). h , i Western blotting analysis of p-eIF2α, eIF2α, FTL, and FTH in colonic epithelial tissue of indicated mice. β-actin was used as the loading control. Statistical analyses were performed with Student’s t tests (two groups) or one-way ANOVA (more than two groups).

Article Snippet: To treat mice with GSK2606414 (GSK 414) in vivo, the mice were administered either GSK 414 (Selleck, Shanghai, China, suspended in vehicle solution containing 0.5% hydoxypropylmethyl cellulose and 0.1% Tween 80 in water at pH 4.8, 50 mg/kg body weight) , or vehicle solution by oral gavage daily during DSS administration.

Techniques: Comparison, Activity Assay, Staining, Labeling, Flow Cytometry, Western Blot, Control

Journal: Laboratory Medicine

Article Title: Variation in LOD Across SARS-CoV-2 Assay Systems: Need for Standardization

doi: 10.1093/labmed/lmaa103

Figure Lengend Snippet: Representative SARS-CoV-2 EUA Assay Systems.

Article Snippet: Gnomegen COVID-19 RT-Digital PCR Detection Kit/Gnomegen Real-Time Digital PCR Instrument or QuantStudio 3D Digital PCR System , RT-digital PCR , N1/N2 , 8 copies/reaction.

Techniques: Luminex, Digital PCR, Real-time Polymerase Chain Reaction, Amplification, CRISPR, Sequencing, Virus

Journal: ACS chemical biology

Article Title: Drug-Like Small Molecules That Inhibit Expression of the Oncogenic MicroRNA-21.

doi: 10.1021/acschembio.2c00502

Figure Lengend Snippet: Figure 5. Anti-proliferative activity of compounds 45 and 52 against gastric (AGS) and pancreatic (ASPC1) cancer cell lines, measured as a function of time in a blind experimental format and compared to the breast cancer drug Palbociclib (Ibrance). Cell viability was assayed using standard MTS assays in the indicated cell lines, following the addition of each compound at 10 nmoles/mL or 10 μM; error bars show experimental uncertainty from results collected in triplicate. Palbociclib and compound 52 reduce the viability of both cell lines to a statistically significant extent (marked with asterisks) while compound 45 does not have any significant effect.

Article Snippet: Palbociclib was purchased from Selleckchem.

Techniques: Activity Assay

Journal: ACS chemical biology

Article Title: Drug-Like Small Molecules That Inhibit Expression of the Oncogenic MicroRNA-21.

doi: 10.1021/acschembio.2c00502

Figure Lengend Snippet: Figure 6. Levels of pre-miR-21 (left) and mature miR-21 (right) measured in gastric adenocarcinoma (AGS) and pancreatic cancer cell lines (ASPC 1), normalized to internal controls (U6 snRNA), in the presence of 10 nmoles/1 mL (10 μM concentration) of compound 45, compound 52, or Palbociclib (Ibrance; used here as a negative control not expected to affect microRNA levels). MicroRNA levels were measured 48 h after incubation, relative to DMSO control, and measured by standard quantitative real-time polymerase chain reaction (qRT-PCR). Statistically significant changes are labeled with asterisks.

Article Snippet: Palbociclib was purchased from Selleckchem.

Techniques: Concentration Assay, Negative Control, Incubation, Control, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Labeling